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Functional changes in mitochondrial properties as a result of their membrane cryodestruction. II. Influence of freezing and thawing on ATP complex activity of intact liver mitochondria |
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| Authors: | T Tsvetkov L Tsonev N Meranzov I Minkov |
| Institution: | 1. Department of Zoology and Entomology, University of Pretoria, Private Bag X20, Hatfield 0028, South Africa;2. Department of Biochemistry, University of Pretoria, Private Bag X20, Hatfield 0028, South Africa;2. Division of Respirology, Neurology, and Rheumatology, Department of Internal Medicine, Kurume University School of Medicine, Kurume, Japan;3. Department of Pathology, Kurume University School of Medicine, Kurume, Japan;4. Center for Clinical and Translational Research, Kyushu University Hospital, Fukuoka, Japan |
| Abstract: | The influence of the freeze-thawing rates on ATP synthetase (ATPase) complex of intact liver mitochondria was investigated. It was shown that the increase in latent ATPase activity and decrease in ATP synthetase activity resulted from an influence on the inner mitochondrial membrane. An increase in freeze-thawing rates led to the preservation of ATP synthetase activity and ATP hydrolysis reduction. Kinetic parameter changes of the ATP synthetase reaction resulted from an insignificant nonspecific increase in the inner mitochondrial membrane permeability and changes in its electrochemical potential level. |
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