An electron spin resonance spin-label study of lipophilin in oriented phospholipid bilayers |
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Authors: | J F Post C Dijkema |
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Institution: | Laboratory of Physical Chemistry, University of Groningen, Nijenborgh 16, 9747 AG Groningen, The Netherlands |
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Abstract: | Model membranes consisting of dimyristoyl phosphatidylcholine and a hydrophobic protein from bovine myelin, lipophilin, were studied using the cholesterol-resembling cholestane ESR spin label. Orientation of the membranes made it possible to deconvolute the spectra into two fractions, one of oriented spin labels reflecting phospholipid bilayer of high order, and one of isotropically tumbling spin labels ascribed to the lipid fraction surrounding the protein molecule (boundary lipid). This isotropic tumbling is different from the behavior of phospholipid molecules near the protein, which retain some degree of order, and indicates that the boundary lipid fraction in our model system forms a rather fluid environment for the protein. A nonlinear relation was found between protein concentration and amount of boundary spin labels. Addition of cholesterol decreases the amount of boundary spin labels. Both findings form evidence for a preferential binding of cholesterol by the membrane protein. |
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Keywords: | To whom correspondence should be addressed at present address: Carnegie-Mellon University Department of Biological Sciences 4400 Fifth Avenue Pittsburgh Pa 15213 |
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