| 10种冬青属植物遗传多样性RAPD和AFLPs分析 |
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| 引用本文： | 钱永生,王慧中,施农农,赵艳,黎念林,胡中. 10种冬青属植物遗传多样性RAPD和AFLPs分析[J]. 分子细胞生物学报, 2008, 41(1): 35-43 |
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| 作者姓名： | 钱永生 王慧中 施农农 赵艳 黎念林 胡中 |
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| 作者单位： | [1]杭州师范大学生物化学与分子生物学杭州市重点实验室,杭州310018 [2]浙江工商大学、生物工程系,杭州310018 [3]杭州植物园环境绿化工程部,杭州310013 |
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| 基金项目： | 浙江省自然科学基金 , 浙江省151基金 , 杭州市131人才基金 |
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| 摘 要： | 采用RAPD和AFLP技术,对10种冬青属植物基因组进行DNA片段扩增,以研究该属种间遗传多样性.结果表明:在RAPD分析中,通过对100种10个碱基随机引物的筛选,发现11种引物能得到多态性较高扩增产物,11种引物共扩增出301条多态性条带,多态率为98.63%.在AFLP分析中,3对选择性引物组合均扩增出了丰富的多态性片段.利用RAPD和AFLP技术分析,结果按UPGMA类平均法进行聚类,聚类结果显示冬青和代茶冬青,木姜冬青和浙江冬青以及光枝刺缘冬青与毛枝三花冬青之间的亲缘关系最近.
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| 关 键 词： | 冬青属 RAPD AFLP 遗传多样性 冬青属植物 遗传多样性 RAPD 技术分析 BASED SPECIES GENETIC DIVERSITY 亲缘关系 浙江 木姜冬青 代茶 显示 聚类 类平均法 UPGMA 利用 引物组合 选择性 扩增产物 多态性 |
| 收稿时间： | 2007-08-24 |
| 修稿时间： | 2007-11-30 |
STUDIES OF GENETIC DIVERSITY AMONG 10 SPECIES OF ILEX BASED ON RAPD AND AFLPs |
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| QIAN Yong Sheng,WANG Hui Zhong,SHI Nong Nong,ZHAO Yan,LI Nian Lin,HU Zhong. STUDIES OF GENETIC DIVERSITY AMONG 10 SPECIES OF ILEX BASED ON RAPD AND AFLPs[J]. Journal of Molecular Cell Biology, 2008, 41(1): 35-43 |
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| Authors: | QIAN Yong Sheng WANG Hui Zhong SHI Nong Nong ZHAO Yan LI Nian Lin HU Zhong |
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| Affiliation: | Key Laboratory of Hangzhou City for Biochemistry and Molecular Biology, Hangzhou Normal University, Hangzhou 310018, China. |
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| Abstract: | The techniques of random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphisms (AFLPs) were used to amplify the genomic DNA of 10 species of Ilex, so as to reveal the polymorphism in different species of Ilex. In RAPD analysis, a total of 301 lines were amplified with 11 kinds of primers selected from 100 kinds of 10 bp random primers, and the polymorphism was 98.63%. In AFLPs analysis,a huge diversity was showed by 3 primers combinations carried out on these species. Both RAPD and AFLPs analysis were clustered by UPGMA and the results showed that the genetic distances were the closest between I. purpurea and I. vomitoria Ait, I. Litseaefolia and I. zhejiangensis, I. hylonoma var. glabra and I. triflora var. kanehirai. |
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| Keywords: | Ilex. RAPD. AFLP. polymorphism |
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