Cloning of a thermostable alpha-amylase gene from Thermomonospora curvata and its expression in Streptomyces lividans |
| |
| Authors: | M Petrícek K Stajner P Tichy |
| |
| Institution: | Department of General Microbiology, Institute of Microbiology, Czechoslovak Academy of Sciences, Prague. |
| |
| Abstract: | The gene from Thermomonospora curvata CCM 3312 coding for thermostable alpha-amylase (tam) has been cloned in Streptomyces lividans TK 24 and localized to a 2.6 kb HindIII-BamHI fragment of DNA. The data presented here show that the tam gene is expressed at a high level in S. lividans and that the protein is efficiently excreted. |
| |
| Keywords: | |
|
