Concentration of live pico- and nanoplankton by means of tangential flow filtration |
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| Authors: | Barthel, Klaus-Gunther Schneider, Gerald Gradinger, Rolf Lenz, Jurgen |
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| Affiliation: | Institut für Meereskunde, Abt. Marine Planktologie Düstembrooker Weg 20, D-2300 Kiel 1, FRG 1Present address: Univ. i Tromsø Marinbiologisk Stasjon, Postboks 2550, N-9001 Tromsø, Norway |
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| Abstract: | A tangential flow filtration system for concentrating live pico-and nanoplankton was tested on natural seawater (<20 µm)from Kiel Bight. Twenty- to thirty-fold concentration of samplesranging from 79 to 152 dm3 was easily achieved, the time necessarydepending on sample volume, pumping pressure and filtrate/retentateratio. Losses of different kinds of cells in relation to theconcentration factor were quantified. Recovery rates were negativelycorrelated with the frequency of sample recirculation throughthe filtration unit. |
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