Isolation and characterization of polyphenol oxidase- and peroxidase-producing <Emphasis Type="Italic">Bacillus</Emphasis> strains from fully fermented tea (<Emphasis Type="Italic">Camellia sinensis</Emphasis>) |
| |
Authors: | Faezi Ghasemi Mohammad Tayeri Alireza |
| |
Institution: | (1) Department of Microbiology, Faculty of Science, Islamic Azad University-Lahijan Branch, Shagaiegh St., P.O. Box 1616, Lahijan, Iran;(2) Department of Microbiology, Faculty of Science, Islamic Azad University-Astara Branch, Astara, Iran |
| |
Abstract: | Sixteen aerobic endospore-forming Bacillus spp. were isolated from fully fermented tea leaf samples from 10 tea factories in Lahijan and Langrod cities (Gillan province,
Iran). Bacillus spp. isolates were characterized using phenotypic characteristics, antibiotic susceptibility and cellular fatty acid (CFA)
patterns. Based on the data obtained, five isolates of tea Bacillus spp. (TB): TB2, TB4, TB6, TB10 and TB12 belonged to the species B. subtilis. Two isolates, TB1 and TB14 were recognized as B.
licheniformis. Two Bacillus spp. isolates, TB9 and TB 16 were identified as B. sphaericus. Two isolates, TB5 and TB13 were shown to be B. pumilus. Two isolates, TB7 and TB15 belonged to B. cereus. Amongst the isolates, Bacillus sp. TB3, Bacillus sp. TB8 and Bacillus sp. TB11 showed different phenotypic traits, distinct antibiotic sensitivity and fatty acid profiles, and they may represent
novel species. The isolates showed polyphenol oxidase (tyrosinase) and peroxidase activities. The highest polyphenol oxidase
and peroxidase activities were observed for Bacillus sp. TB3 and B. licheniformis TB14, respectively, where values of 5.48 and 3.73 units mL−1 were observed. |
| |
Keywords: | Bacillus spp Camellia sinensis Characterization Peroxidase Polyphenol oxidase |
本文献已被 SpringerLink 等数据库收录! |
|