Strong and regulated promoters in the cyanobacterium Anabaena PCC 7120 |
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Authors: | Jeff Elhai |
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Affiliation: | MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA |
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Abstract: | Abstract The strengths of several promoters were assessed in the cyanobacterium Anabaena PCC 7120 by fusing them to luxAB , encoding bacterial luciferase. Two promoters, P tac and P psbA , with sequences nearly identical to consensus Escherichia coli σ 70 promoters, gave as high or higher expression than the strong Anabaena promoter, P rbc . P npt , the natural promoter driving expression of the kanamycin-resistance determinant from Tn5, was poorly expressed in Anabaena . The Lac repressor partially repressed expression from P tac , permitting regulated expression in Anabaena after induction with isopropyl thiogalactoside to a level 4–5-fold higher than without inducer. |
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Keywords: | Cyanobacteria Promoter Luciferase Anabaena Lac repressor Gene expression vectors |
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