Extraction and identification by mass spectrometry of undecaprenyl diphosphate-MurNAc-pentapeptide-GlcNAc from Escherichia coli |
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| Authors: | Guan Ziqiang Breazeale Steven D Raetz Christian R H |
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| Affiliation: | Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA. zguan@biochem.duke.edu |
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| Abstract: | Undecaprenyl diphosphate-MurNAc-pentapeptide-GlcNAc (lipid II) is extracted from Escherichia coli cells by utilizing its unusual pH-dependent solubility property in a Bligh-Dyer system, and identified by electrospray ionization mass spectrometry in conjunction with a novel 15N mass shift analysis. The described approach will facilitate the structural characterization of lipid II variants from diverse bacteria, including antibiotic-resistant mutants, as well as the numerous minor uncharacterized lipids present in all biological systems. |
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| Keywords: | Lipid II Escherichia coli Mass spectrometry 15N mass shift analysis |
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