Electrostatic modulation of electron transfer in the active site of heme peroxidases |
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Authors: | Gábor Náray-Szabó |
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Institution: | Department of Theoretical Chemistry, Loránd E?tv?s University, Pázmány Péter st. 2, H-1117 Budapest, Hungary?,e-mail: naray@para.chem.elte.hu, HU
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Abstract: | The heme enyzmes cytochrome c peroxidase (CCP) and pea cytosolic ascorbate peroxidase (APX) show a high level of sequence identity. The main difference
near the active sites is the presence of a cation binding site in APX located about 1 nm from the Trp-179 side chain, which
is hydrogen-bonded to Asp-208. It is possible that this difference in electrostatics provided by the protein environment is
an essential determinant of the stabilization of the ion-pair or neutral form of the Trp...Asp couple in APX and CCP. Semiempirical
molecular orbital calculations support the hypothesis that the position of the moving proton inside the couple influences
the location of the free electron, leading to radical formation either on the heme or on the Trp side chain of these enzymes.
Received, accepted: 26 November 1996 |
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Keywords: | Heme peroxidases Protein electrostatics Free radical location Molecular orbital calculations |
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