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Mode of increase in cell wall polysaccharides in synchronously growing Saccharomyces cerevisiae
Authors:Masaya Hayashibe  Noriyuki Abe  Motoi Matsui
Affiliation:(1) Laboratory of Applied Microbiology, Faculty of Agriculture, Yamagata University, 997 Tsuruoka-shi, Yamagata-ken, Japan;(2) Present address: Department of Biology, Faculty of Science, Osaka City University, Sumiyoshi-ku, 558 Osaka, Japan
Abstract:The mode of increase in cell wall polysaccharides of yeast (glucan and mannan) during cell cycle was analyzed using cell wall samples obtained from a synchronous culture. The increase in mannan and total glucan proceeded almost linearly throughout the cell cycle except for a short period of their leveling off at the time of cell division. However, the constituents of glucan behaved characteristically: Alkalisoluble glucan and insoluble glucan increased mainly in the former and the latter half of the cell cycle, respectively.
Keywords:Saccharomyces cerevisiae  Cell wall  Glucan  Mannan  Synchronous culture
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