Comparison of the laccases, molecular marker proteins, and induction of pycnidia by three species of botryosphaeriaceous fungi |
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Authors: | Ana-Flora D Vasconcelos Robert F H Dekker Aneli M Barbosa Luzia Paccola-Meirelles |
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Institution: | (1) Biologia Geral (CCB), Universidade Estadual de Longdrina, CEP 86051-990, Caixa Postal 6001, Londrina, Paraná, Brazil;(2) Technologia de Alimentos e Medicamentos (CCA), Universidade Estadual de Londrina, CEP 86051-990, Caixa Postal 6001, Londrina, Parana, Brazil;(3) Bioquímica (CCE), Universidade Estadual de Londrina, CEP 86051-990, Caixa Postal 6001, Londrina, Paraná, Brazil |
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Abstract: | Three species of botryosphaeriaceous fungi,Botryosphaeria sp. isolate MAMB-5,Botryosphaeria ribis andLasiodiplodia theobromae, were compared for the production of pycnidia and laccases. Laccases were produced both intra- and extra-cellularly when
the fungi were cultivated on basal medium in the presence and absence of veratryl alcohol, withBotryosphaeria sp. MAMB-5 showing the highest enzyme titres. Electrophoretic examination of intracellular marker proteins (esterases and
phosphatases) and laccases indicated that the three species were genetically distinctly different, although the laccase zymograms
for the three fungi showed similarity. The production of pycnidia occurred under continuous lighting at 28°C, but conditions
differed among the three fungal species. Production could be induced on artificial media (potato-dextrose and oat agar) under
stress-induced conditions where the mycelium was stimulated by physical abrasion, and in the case ofBotryosphaeria sp. isolate MAMB-5 on eucalypt woodchips. Evidence is presented that veratryl alcohol facillitated the secretion of intracellular-localised
laccases into the extracellular medium. |
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Keywords: | Botryosphaeria species laccases Lasiodiplodia theobromae pycnidium formation veratry alcohol |
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