Cryopreservation of fetal rat brain tissue later used for intracerebral transplantation |
| |
| Authors: | S Jensen T S?rensen J Zimmer |
| |
| Affiliation: | 1. Neuroscience Research Group, University of Saskatchewan, Saskatoon, SK S7N 5E5, Canada;2. College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, SK S7N 5E5, Canada;3. Department of Geological Sciences, University of Saskatchewan, Saskatoon, SK S7N 5E5, Canada;2. GRECC, MERCE, Baltimore, Maryland;3. Department of Neurology, University of Maryland School of Medicine, Baltimore, Maryland;1. Department of Epidemiology, School of Public Health, Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, Medical College of Soochow University, Suzhou, Jiangsu, China;2. Department of Epidemiology, School of Public Health, Guizhou Medical University, Guiyang, China;3. Department of Neurology, Affiliated Hospital of Hebei United University, Hebei, China;4. Department of Neurology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China;5. Department of Epidemiology, Tulane University School of Public Health and Tropical Medicine, New Orleans, LA, USA;6. Department of Cardiology, the First Affiliated Hospital of China Medical University, Liaoning, China;7. Department of Medicine, Tulane University School of Medicine, New Orleans, LA, USA;1. Environment & Life Sciences Graduate Program, Trent University, 1600 West Bank Drive, Peterborough, ON K9L 0G2, Canada;2. School of the Environment, Trent University, 1600 West Bank Drive, Peterborough, ON K9L 0G2, Canada;3. Water Quality Centre, Trent University, 1600 West Bank Drive, Peterborough, ON K9L 0G2, Canada |
| |
| Abstract: | Intracerebral grafting of immature brain tissue is now widely used as a tool to study neuronal development and regeneration in the brain and spinal cord. This has stimulated the interest in methods for storage of such tissue before transplantation. In this study a method for cryopreservation of immature rat central nervous tissue is presented and discussed in relation to current cryobiological principles. The method was applied to brain tissue from 16- and 17-day-old fetal rats, including the neocortex, habenula, septum and basal forebrain, cerebellum, and retina. After storage in liquid nitrogen from 6 to 52 days the tissue was grafted into the brain of adult rats. The recipients survived for 23 to 673 days before their brains were processed by current neuroanatomical, histological methods. The presence of graft tissues was recorded and their cellular and connective organization was examined, including their exchange of nerve connections with the host brain. The results obtained were comparable with results from other studies where the same tissues were grafted immediately after removal from the donor, and a study of cryopreservation of developing hippocampal tissue. We conclude that cryopreservation is a reliable method for storage of immature neural tissue later to be used for intracerebral grafting. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
