Bax does not directly participate in the Ca(2+)-induced permeability transition of isolated mitochondria |
| |
Authors: | De Marchi Umberto Campello Silvia Szabò Ildikò Tombola Francesco Martinou Jean-Claude Zoratti Mario |
| |
Institution: | CNR Institute of Neuroscience, Biomembranes Section and Department of Biomedical Sciences, University of Padova, Padova, Italy. |
| |
Abstract: | The mitochondrial permeability transition pore and Bax have both been proposed to be involved in the release of pro-apoptotic factors from mitochondria in the "intrinsic" pathway of apoptosis. The permeability transition pore is widely thought to be a supramolecular complex including or interacting with Bax. Given the relevance of the permeability transition in vivo, we have verified whether Bax influences the formation and/or the properties of the Ca(2+)/P(i)-induced permeability transition by using mitochondriaisolated from isogenic human colon cancer bax(+/-) and bax(-/-) HCT116 cell lines. We used mitochondria isolated from both types of cells and from Bax(+) cells exposed to apoptotic stimuli, as well as Bax-less mitochondria into which exogenous Bax had been incorporated. All exhibited the same behavior and pharmacological profile in swelling and Ca(2+)-retention experiments. Mitochondria from a bax(-)/bak(-) cell line also underwent an analogous Ca(2+)/P(i)-inducible swelling. This similarity indicates that Bax hasno major role in regulating the Ca(2+)-induced mitochondrial permeability transition. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|