首页 | 本学科首页   官方微博 | 高级检索  
     


Coccidioidomycosis and blastomycosis: Advances in molecular diagnosis
Authors:Ralf Bialek  Gloria M. González  Dominik Begerow  Ulrike E. Zelck
Affiliation:1. Institut für Tropenmedizin, Universitätsklinikum Tübingen, Keplerstrasse 15, D-72074 Tübingen, Germany;2. Departamento de Microbiología, Facultad de Medicina, Universidad Autónoma de Nuevo Léon, Monterrey, Nuevo Léon, México;3. Institute of Biology I, University of Tübingen, Tübingen, Germany;1. Department of Internal Medicine, Infectious Diseases Division, Washington University, School of Medicine Campus Box 8051, 660 S. Euclid Ave., St. Louis Missouri 63110-1093 USA. Tel.: +1 40 61 35 18; fax: +1 40 61 34 19;2. Laboratorio de Inmunología de Hongos, Depto. de Microbiología y Parasitología, Facultad de Medicina, UNAM Cd. Universitaria 04510 México, D.F. Tel.:/fax: +52 555 56-23-24-62;3. Lille-2 University Hospital Center and Ecology of Parasitism, Institute Pasteur of Lille 1, rue du Prof-Calmette - BP245 59019 Lille cedex (France) Tel.: +33 3 20 87 71 55; fax: +33 3 20 87 72 24
Abstract:Clinical isolates of Coccidioides spp. and Blastomyces dermatitidis can be identified by chemiluminescent DNA probes and PCR assays targeting multicopy genes. In fixed tissue samples, cells of the two fungi are specified by in situ hybridization and PCR assays targeting 18S rDNA but sequencing of the products is mandatory. Nested PCR assays targeting genes encoding species- or genus-specific proteins like proline rich antigen of Coccidioides spp. and B. dermatitidis adhesin facilitate amplification of specific DNA from fixed tissue samples. The value of DNA amplification from native specimens of suspected cases of coccidioidomycosis or blastomycosis still needs to be determined.
Keywords:
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号