Electrophoresis karyotype and chromosome-length polymorphism of Histoplasma capsulatum clinical isolates from Latin America |
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| Authors: | Cristina Elena Canteros María Fernanda Zuiani Viviana Ritacco Diego E Perrotta María Rocío Reyes-Montes Julio Granados Gerardo Zúñiga Maria Lucia Taylor Graciela Davel |
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| Institution: | 1. Departamento Micolog??a, INEI ANLIS “Dr. Carlos G. Malbrán”, Av. Velez Sarsfield 563, 1281 Buenos Aires, Argentina;2. Consejo Nacional de Investigaciones Cient??ficas y Técnicas (CONICET), Buenos Aires, Argentina;3. Departamento de Microbiolog??a-Parasitolog??a, Facultad de Medicina, Universidad Nacional Autónoma México, México DF, 04510, Mexico;4. Instituto Nacional de Ciencias Médicas y Nutrición “Salvador Zubirán”, México DF, 14000, Mexico;5. Departamento de Zoolog??a, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México DF, 11340, Mexico;1. Department of Internal Medicine, Infectious Diseases Division, Washington University, School of Medicine Campus Box 8051, 660 S. Euclid Ave., St. Louis Missouri 63110-1093 USA. Tel.: +1 40 61 35 18; fax: +1 40 61 34 19;2. Laboratorio de Inmunología de Hongos, Depto. de Microbiología y Parasitología, Facultad de Medicina, UNAM Cd. Universitaria 04510 México, D.F. Tel.:/fax: +52 555 56-23-24-62;3. Lille-2 University Hospital Center and Ecology of Parasitism, Institute Pasteur of Lille 1, rue du Prof-Calmette - BP245 59019 Lille cedex (France) Tel.: +33 3 20 87 71 55; fax: +33 3 20 87 72 24 |
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| Abstract: | Intact chromosomes of 19 clinical isolates of Histoplasma capsulatum recently obtained in Argentina, Mexico and Guatemala and the laboratory reference strain G186B from Panama were analyzed using pulsed-field gel electrophoresis. Chromosomal banding patterns of the human isolates revealed 5–7 bands, ranging from 1.3 to 10 Mbp in size. Strain G186B showed five bands of approximately 1.1, 2.8, 3.3, 5.4 and 9.7 Mbp. Thirteen different electrokaryotypes were identified, indicating that the genome of H. capsulatum varies widely in nature, as observed previously in laboratory strains. No definite association was found between electrokaryotype and geographical or clinical source. |
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