Genotypic diversity and virulent factors of Staphylococcus epidermidis isolated from human breast milk |
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| Authors: | Jelena Begović Branko Jovčić Milena Papić-Obradović Katarina Veljović Jovanka Lukić Milan Kojić Ljubiša Topisirović |
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| Affiliation: | 1. Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Laboratory for Molecular Genetics of Industrial Microorganisms, Vojvode Stepe 444a, P.O. Box 23, 11010 Belgrade, Serbia;2. University of Belgrade, Faculty of Biology, Studentski trg 16, 11000 Belgrade, Serbia;3. Gynecology and Obstetrics Clinic, Narodni Front, School of Medicine, University of Belgrade, Kraljice Natalije 62, 11010 Belgrade, Serbia |
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| Abstract: | Staphylococcus epidermidis strains were isolated from the expressed human breast milk (EHM) of 14 healthy donor mothers. Genetic diversity was evaluated using RAPD-PCR REP-PCR and pulse-field gel electrophoresis (PFGE). PFGE allowed the best discrimination of the isolates, since it provided for the greatest diversity of the analyzed genomes. Among the S. epidermidis strains, resistance to gentamicin, tetracycline, erythromycin, clindamycin or vancomycin was detected, whilst four isolates were multiresistant. The results from our study demonstrate that staphylococci from EHM could be reservoirs of resistance genes, since we showed that tetK could be transferred from EHM staphylococci to Gram-negative Escherichia coli. Most of the staphylococcal strains displayed excellent proteolytic and lipolytic activities. Additionally, the presence of ica genes, which was related to their ability to form a biofilm on tissue culture plates, and the presence of virulence factors including autolysin/adhesin AtLE, point to their pathogenic potential. |
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