Analysis and expression of STE13ca gene encoding a putative X-prolyl dipeptidyl aminopeptidase from Candida albicans |
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| Authors: | Consuelo Bautista-Muñoz César Hernández-Rodr??guez Lourdes Villa-Tanaca |
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| Institution: | 1. Department of Internal Medicine, Infectious Diseases Division, Washington University, School of Medicine Campus Box 8051, 660 S. Euclid Ave., St. Louis Missouri 63110-1093 USA. Tel.: +1 40 61 35 18; fax: +1 40 61 34 19;2. Laboratorio de Inmunología de Hongos, Depto. de Microbiología y Parasitología, Facultad de Medicina, UNAM Cd. Universitaria 04510 México, D.F. Tel.:/fax: +52 555 56-23-24-62;3. Lille-2 University Hospital Center and Ecology of Parasitism, Institute Pasteur of Lille 1, rue du Prof-Calmette - BP245 59019 Lille cedex (France) Tel.: +33 3 20 87 71 55; fax: +33 3 20 87 72 24 |
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| Abstract: | Candida albicans STE13ca gene was identified by its homology to the Saccharomyces cerevisiae STE13 gene that encodes for the dipeptidyl aminopeptidase A (DAP A) involved in the maturation of α-factor mating pheromone. Our study revealed that C. albicans ATCC 10231 depicts dipeptidyl aminopeptidase activity. We also analyzed the expression of the STE13ca gene homologue from this pathogenic yeast. This gene of 2793 pb is homozygotic and encodes for a predicted protein of 930 amino acids with a molecular weight of 107,035 Da. The predicted protein displays significant sequence similarity to S. cerevisiae Ste13p. This C. albicans gene is located in chromosome R. STE13ca gene increases its levels of expression in conditions of nutritional stress (proline as nitrogen source) and during formation of the germinal tube, suggesting a basic biological function for the STE13ca in this yeast. |
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