Quantitative measurement of starch in very small amounts of leaf tissue |
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Authors: | J. C. MacRae |
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Affiliation: | (1) Applied Biochemistry Division, D.S.I.R., Palmerston North, New Zealand |
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Abstract: | Summary A specific enzyme method is described for the routine estimation of starch in small quantities (10–30 mg) of dried leaf tissue. A -glucanase-free preparation of amyloglucosidase is employed to hydrolyse starch to glucose; this is subsequently estimated by the glucose oxidase technique. The method gives result which agree closely with those obtained by a specific iodine-precipitation method. |
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