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Formation of phosphatidic acid, ceramide, and diglyceride on radiolysis of lipids: identification by MALDI-TOF mass spectrometry |
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| Authors: | Shadyro Oleg Yurkova Irina Kisel Mikhail Brede Ortwin Arnhold Jürgen |
| Institution: | * Department of Chemistry, Belarusian State University, Minsk, Belarus ? Institute of Bioorganic Chemistry, Belarus National Academy of Sciences, Minsk, Belarus ? Research Unit Time-Resolved Spectroscopy, University of Leipzig, Leipzig, Germany § Institute of Medical Physics and Biophysics, Medical Department, University of Leipzig, Leipzig, Germany |
| Abstract: | By use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, phosphatidic acid was found to be the main product of γ radiolysis of cardiolipin, phosphatidylinositol, and phosphatidylglycerol. It has been shown that γ irradiation of such glycolipids as cerebroside and galactosyl diglyceride leads to formation of ceramide and diglyceride, respectively. These findings, combined with those obtained earlier, allowed an assumption to be made that, owing to radiation-induced free radical fragmentation of lipids in their polar moiety, formation of signaling molecules can occur. |
| Keywords: | Phospholipids Glucosylceramide Galactosyl diglyceride γ radiolysis Matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy Fragmentation Free radicals Phosphatidic acid Ceramide Diglyceride |
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