Isolation of a cDNA encoding the human homolog of COX17, a yeast gene essential for mitochondrial copper recruitment |
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Authors: | Ravi Amaravadi D Moira Glerum A Tzagoloff |
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Institution: | (1) Department of Biological Sciences, Columbia University, New York, NY 10027, USA Tel.: +1-212-854-2920; Fax: +1-212-865-8246, US |
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Abstract: | The COX17 gene of Saccharomyces cerevisiae codes for a cytoplasmic protein essential for the expression of functional cytochrome oxidase. This protein has been implicated
in targeting copper to mitochondria. To determine if Cox17p is present in mammalian cells, a yeast strain carrying a null
mutation in COX17 was transformed with a human cDNA expression library. All the respiratory competent clones obtained from the transformations
carried a common cDNA sequence with a reading frame predicting a product homologous to yeast Cox17p. The cloning of a mammalian
COX17 homolog suggests that the encoded product is likely to function in copper recruitment in eucaryotic cells in general. Its
presence in humans provides a possible target for genetically inherited deficiencies in cytochrome oxidase.
Received: 22 August 1996 / Revised: 31 October 1996 |
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