A Dithiothreitol-Sensitive Tetrameric Protease from Spinach Thylakoids Has Polyphenol Oxidase Activity |
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Authors: | Kuwabara Tomohiko; Masuda Tetsuya; Aizawa Sachiko |
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Institution: | 1 Institute of Biological Science, University of Tsukuba Tsukuba, Ibaraki, 305 Japan
2 Master's Program in Biosystem Studies, University of Tsukuba Tsukuba, Ibaraki, 305 Japan |
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Abstract: | Polyclonal antibody raised against a dithiothreitol-sen-sitivetetrameric protease (DSTP) from PSII membranes specificallyinhibited the polyphenol oxidase (PPO) activity of spinach thylakoids.DSTP was copurified with PPO activity on an affinity columnprepared with antibody against DSTP. These results suggest thatDSTP and PPO are the same protein. During purification of DSTP,Tween 20 was essential for stabilization of the protein, whichwas degraded in the absence of the detergent. Gel-filtrationchromatog-raphy of the purified DSTP revealed the presence of230-kDa (tetramer) and 60-kDa (monomer) species. The coppercontent of monomer species was determined to be 0.4 Cu atomper protein molecule, when the molecular weight of the proteinwas calculated to be 62,243, which is the value reported forspinach PPO Hind et al. (1995) Biochemistry 34: 8157]. PurifiedDSTP caused the degradation as well as the dimerization of theextrinsic 23-kDa protein of PSII. The degradation of the proteinwas suppressed under anaerobic conditions induced by the presenceof glucose oxidase and glucose together. This fact suggeststhat oxygen molecules are involved in the proteolytic reactionand that the proteolytic activity and PPO activity may be correlatedwith each other. (Received September 27, 1996; Accepted December 4, 1996) |
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