Effects of Nickel on Human and Fish Red Blood Cells |
| |
Authors: | Grazia De Luca Tiziana Gugliotta Giulia Parisi Pietro Romano Antonella Geraci Orazio Romano Adriana Scuteri Leonardo Romano |
| |
Institution: | (1) Dipartimento di Fisiologia Generale e Farmacologia, Facoltà di Scienze MM.FF.NN, Università degli studi di Messina, Via Salita Sperone, Ctr. Papardo, Papardo, Messina, 98166, Italy;(2) Dipartimento di Scienze Biochimiche, Fisiologiche e della Nutrizione, Facoltà di Medicina e Chirurgia, Università degli studi di Messina, Papardo, Messina, Italy |
| |
Abstract: | The objective of this study was to assess the effects of nickel chloride on human and rainbow trout erythrocytes in vitro.
The cells were incubated with 0, 0.5 and 1 mM nickel chloride for 1 h at pH 7.40 and 25°C, then K+ efflux, SO42− uptake and GSH and GSSG concentrations were measured. In both kind of cells, “high concentration” nickel treatment increased
KCl efflux with respect to the control. The SO42− uptake was not significantly different at “low nickel concentration” but was lower in erythrocytes treated with 1 mM nickel
chloride; the rate constant of SO42− uptake decreased by 35% in human erythrocytes and by 44% in fish erythrocytes. Nickel chloride also acts on cellular metabolism
and in particular on erythrocyte glutathione peroxidase with consequent increase in oxidative stress; the data show a significant
decrease in intracellular GSH in both human (25%) and fish erythrocytes (18%) after treatment with nickel chloride, with concomitantly
high GSSG concentrations and lower GSH/GSSG ratios. |
| |
Keywords: | Erythrocytes Nickel chloride GSH Anion transport |
本文献已被 PubMed SpringerLink 等数据库收录! |
|