Confirmation and refinement of the localisation of the c-MEL locus on chromosome 19 by physical and genetic mapping |
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| Authors: | E Nimmo R -A Padua D Hughes J D Brook R Williamson K J Johnson |
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| Institution: | (1) Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, Paddington, W2 1PG London, UK;(2) LRF Preleukaemia Unit, Department of Haematology, University Hospital of Wales, Heath Park, CF4 4XN Cardiff, UK;(3) Institute of Medical Genetics, University of Wales College of Medicine, Heath Park, CF4 4XN Cardiff, UK |
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| Abstract: | Summary The human gene locus c-MEL was identified following transfection of genomic DNA from the human melanoma cell line NK14; it
has previously been assigned to chromosome 19 (p13.2–q13.2) by analysis of somatic cell hybrids. We have further refined the
position of this gene to the proximal region of 19p (cen-p13.2), using cell hybrids containing only fragments of human chromosome
19. We have confirmed this physical localisation by linkage analysis with a recently described restriction fragment length
polymorphism for the c-MEL gene, and mapped the locus within the region of the low density lipoprotein receptor gene (LDLR)
(Lod 4.43,
) and the anonymous marker D19S11 (13.1.25) (Lod 9.33,
). This gene thus maps to a region of chromosome 19 involved in karyotypic abnormalities in a variety of malignancies including
melanomas and leukaemias. |
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