| Candida cloacae长链脂肪酸醇氧化酶基因的克隆与表达 |
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| 引用本文： | 葛正龙,Antoni R.SLABAS. Candida cloacae长链脂肪酸醇氧化酶基因的克隆与表达[J]. 中国生物化学与分子生物学报, 2003, 19(2): 264-267 |
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| 作者姓名： | 葛正龙 Antoni R.SLABAS |
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| 作者单位： | 1. 贵州省遵义医学院生物化学教研室,遵义,563003
2. 英国Durham大学生物科学系, Durham DH 3LE,英国 |
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| 基金项目： | 国家留学基金委资助项目~~ |
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| 摘 要： | Ｃａｎｄｉｄａｃｌｏａｃａｅ是利用链烃和长链脂肪酸作为碳源来生长的一种工业酵母 .长链脂肪酸在单加氧酶作用下 ,使远离羧基的甲基羟化 ,生成ω 羟脂肪酸 .后者在生物体中通过两条连续的氧化通路进行氧化 (ω氧化通路和 β氧化通路 ) .醇氧化酶是ω氧化通路中的重要组成酶 ,它可以催化链烃或长链脂肪酸分子中的羟基氧化为醛基 ,后者再经其它酶氧化为羧基 .长链脂肪酸通过ω氧化通路生成二羧基化合物 ,它可作为重要的化工原料 ,广泛应用于香料、多聚酰胺、多聚酯、胶类和环内酯抗生素的生成[1] .ω氧化通路中产生的羧基化合物再经 β 氧…
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| 收稿时间： | 2003-04-20 |
| 修稿时间： | 2002-07-09 |
Cloning and Expression of Long-chain Fatty-acid Alcohol Oxidase from Candida cloacae |
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| GE Zheng-long ),Antoni R.SLABAS ). Cloning and Expression of Long-chain Fatty-acid Alcohol Oxidase from Candida cloacae[J]. Chinese Journal of Biochemistry and Molecular Biology, 2003, 19(2): 264-267 |
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| Authors: | GE Zheng-long ) Antoni R.SLABAS ) |
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| Affiliation: | ( 1)Department of Biochemistry, Zunyi Medical College, Zunyi 563003, China; 2)Department of Biological Sciences, University of Durham, South Road, Durham DH 3LE, United Kingdom |
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| Abstract: | The cDNA encoding long-chain fatty-acid alcohol oxidase(FAO) was isolated by using RT-PCR method from Candida cloacae grown in a medium containing oleic acid. The cDNA of fatty-acid alcohol oxidase was cloned into expression plasmid pET17b under T7 promoter and then transformed into E.coli BL21/DE3. The molecular weight of the expressed protein was estimated to be approximately 64 kD by SDS-PAGE. The expressed protein had a specific catalytic activity of fatty-acid alcohol oxidase and its activity was 1090±116 U/ml medium. Northern blotting revealed that there was high expression of fao mRNA in Candida cloacae yeast grown in a medium containing oleic acid. |
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| Keywords: | Candida cloacae fatty-acid oxidase gene cloning gene expression oleic acid |
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