Enantioselective synthesis of (S)-2-amino-4-phenylbutanoic acid by the hydantoinase method |
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Authors: | Lo Hsueh-Hsia Kao Chao-Hung Lee Dong-Sheng Yang Teng-Kuei Hsu Wen-Hwei |
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Affiliation: | Institute of Molecular Biology, National Chung-Hsing University, Taichung, Taiwan. |
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Abstract: | Biosynthesis of (S)-(+)-2-amino-4-phenylbutanoic acid (1) was performed by nonenantioselective hydantoinase and L-N-carbamoylase using racemic 5-[2-phenylethyl]-imidazolidine-2,4-dione (rac-2) as a substrate. The compounds involved in this biocatalysis process could be simultaneously resolved by high-performance liquid chromatography using Chirobiotic T column with a mobile phase of EtOH/H(2)O = 10/90 at pH 4.2-4.5. To our knowledge, this is the first report of the successful production of 1 by the combination of recombinant hydantoinase and L-N-carbamoylase. |
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Keywords: | 5‐[2‐phenylethyl]‐imidazolidine‐2,4‐dione (S)‐2‐amino‐4‐phenyl‐butanoic acid hydantoinase L‐N‐carbamoylase HPLC |
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