Relationship between rate of gluconeogenesis and content of nicotinamide adenine dinucleotide in renal cortex |
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Authors: | S Y Ou S A Kempson T P Dousa |
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Affiliation: | Mayo Clinic and Foundation, Department of Physiology, and Division of Nephrology, Rochester, MN 55901, USA |
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Abstract: | Gluconeogenesis in rat renal cortex was measured using tissue slices incubated with or without appropriate substrates. Immediately after incubation the tissue slices were snap-frozen and the content of oxidized nicotinamide adenine dinucleotide (NAD+) was determined. Incubation with 10 mM α-ketoglutarate or L-glutamate led to enhanced glucose production and an increase in tissue content of NAD+. Quinolinate and 3-mercaptopicolinate inhibited the rate of gluconeogenesis from L-glutamate and α-ketoglutarate respectively, and decreased the tissue levels of NAD+. The enhanced rate of gluconeogenesis was associated with an increase of NAD+ in the cytosol fraction (105 × g supernatant) but not in the particulate fraction (105 × g pellet) of renal cortex homogenate. Present results indicate that NAD+ content changes in parallel with the rate of gluconeogenesis in renal cortical tissue. |
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