Regeneration of Indian ginseng plantlets from stem callus |
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Authors: | Manickam V.S. Elango Mathavan R. Antonisamy R. |
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Affiliation: | (1) Centre for Biodiversity and Biotechnology, St. Xavier's College (Autonomous), Palayamkottai, 627 002, India |
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Abstract: | The development of stem callus mediated plant regeneration system for Withania somnifera is described. Maximum callus proliferation was obtained on Murashige and Skoog medium supplemented with 2.26 μM 2,4-D. Three-week-old, white, friable callus was used for shoot regeneration. The maximum shoot regeneration (6.2 ± 0.34 shoots/explant) was achieved in four weeks when callus was cultured on MS medium fortified with 4.44 μM BA and 0.57 μM IAA. Regenerated shoots were excised and multiplied (8.4 ± 0.43 shoots/explant) on MS medium supplemented with 4.44 μM of BA. Multiple shoots were divided into single shoots and were rooted (5.1 ± 0.49 rootlets/shoot) on half strength MS medium supplemented with 9.84 μM of IBA. After a hardening phase of 3 weeks the plantlets were transferred to the field. This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | medicinal plant shoot organogenesis Withania somnifera |
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