The aurea mutant of tomato is deficient in spectrophotometrically and immunochemically detectable phytochrome |
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Authors: | B. M. Parks A. M. Jones P. Adamse M. Koornneef R. E. Kendrick P. H. Quail |
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Affiliation: | (1) Botany Department, University of Wisconsin, 53706 Madison, WI, USA;(2) Laboratory of Plant Physiological Research, Agricultural University, Generaal Foulkesweg 72, 6703 BW Wageningen, Netherlands;(3) Department of Genetics, Agricultural University, Generaal Foulkesweg 53, 6703 BM Wageningen, Netherlands;(4) Present address: Department of Biology, University of North Carolina, 27514 Chapel Hill, NC, USA |
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Abstract: | The aurea locus mutant (auw) of tomato contains less than 5% of the level of phytochrome in wild-type tissue as measured by in vivo difference spectroscopy. Immunoblot analysis using antibodies directed against etiolated-oat phytochrome demonstrates that crude extracts of etiolated mutant tissue are deficient in a major immunodetectable protein (116 kDa) normally present in the parent wild type. Analyses of wild-type tissue extracts strongly indicate that the 116-kDa protein is phytochrome by showing that this protein: a) is degraded more rapidly in vitro after a brief far-red irradiation than after a brief red irradiation (Vierstra RD, Quail PH, Planta 156: 158–165, 1982); b) contains a covalently bound chromophore as detected by Zn-chromophore fluorescence on nitrocellulose blots; and c) has an apparent molecular mass comparable to phytochrome from other species on size exclusion chromatography under non-denaturing conditions. The demonstration that the aurea mutant is deficient in this 116-kDa phytochrome indicates that the lack of spectrally detectable phytochrome in this mutant is the result of a lesion which affects the abundance of the phytochrome molecule as opposed to its spectral integrity. |
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Keywords: | difference spectroscopy phytochrome Western analysis |
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