<Emphasis Type="Italic">Streptomyces</Emphasis> and <Emphasis Type="Italic">Saccharopolyspora</Emphasis> hosts for heterologous expression of secondary metabolite gene clusters |
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Authors: | Richard H Baltz |
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Institution: | (1) CognoGen Biotechnology Consulting, 6438 North Olney Street, Indianapolis, IN 46220, USA |
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Abstract: | Natural products discovery from actinomycetes has been on the decline in recent years, and has suffered from a lack of innovative
ways to discover new secondary metabolites within a background of the thousands of known compounds. Recent advances in whole
genome sequencing have revealed that actinomycetes with large genomes encode multiple secondary metabolite pathways, most
of which remain cryptic. One approach to address the expression of cryptic pathways is to first identify novel pathways by
bioinformatics, then clone and express them in well-characterized hosts with known secondary metabolomes. This process should
eliminate the tedious dereplication process that has hampered natural products discovery. Several laboratory and industrial
production strains have been used for heterologous production of secondary metabolite pathways. This review discusses the
results of these studies, and the pros and cons of using various Streptomyces and one Saccharopolyspora strain for heterologous expression. This information should provide an experimental basis to help researchers choose hosts
for current application and future development to express heterologous secondary metabolite pathways in yields sufficient
for rapid scale-up, biological testing, and commercial production. |
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