Formation of virus-like particles from O-type foot-and-mouth disease virus in insect cells using codon-optimized synthetic genes |
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| Authors: | Yimei Cao Pu Sun Yuanfang Fu Xingwen Bai Feipen Tian Xiangtao Liu Zengjun Lu Zaixin Liu |
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| Institution: | (1) Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science, State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Xujiaping No. 1, Yanchangpu, Lanzhou, 730046, Gansu, The People’s Republic of China; |
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| Abstract: | A recombinant baculovirus was constructed to simultaneously express codon-optimized virus-like particles (VLP), A VP1-2A-VP3
and VP0 of serotype O foot-and-mouth disease virus (FMDV), from individual promoters. The target proteins were expressed in
insect cells at high level, as shown by indirect sandwich ELISA; and the expressed VP1-2A-VP3 could autocatalytically be cleaved
into the individual proteins, VP1-2A and VP3, as shown by Western-blot analyses. In addition, in the insect cells, the structural
proteins, VP0, VP3 and VP1-2A, self-assembled into virus-like particles resembling the authentic FMDV particles. This information
should prove useful for the development of more efficient VLP assembly using shorter genes. |
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