农杆菌属介导的植物细胞遗传转化研究现状

自20实际70年代末提出农杆菌属介导产生转基因植物以来,已相继有很多农作物和园艺植物被农杆菌成功转化。在一些发达国家已有大量种植的转基因经济作物如玉米、大豆、棉花、马铃薯、番茄等。但越来越多的经济作物以及园林树木和园艺植物对粒子轰击等转化方法无效或效果不佳,因而农杆菌因其介导的遗传转化率较高就越来越受到重视。但是,农杆菌介导的转基因在表达稳定性和可预见性方面仍然存在诸多问题。本文介绍了有关农杆菌分子生物学的基础研究及提高农杆菌介导的遗传转化效率的研究进展。

Researches on Agrobacterium-Mediated Transformation of Plant Cells

Since the idea of producing transgenic plants by Agrobacterium-mediated transformation in 1970s many crops and horticulture plants had been successfully transformed by Agrobacterium large scale of which were being widely grown now. Moreover more and more scientists take attention to Agrobacterium because of its higher transformation efficiency and few successes of other methods such as particle bombard and so on. However there are still some problems in the stability and prodictibility of gene expression transformed by Agrobacterium. So in recent years many national rearches focus on the molecular bases of Agrobacterium biology and ways to improve efficiency of Agrobacterium-mediated transformation. This paper makes a summary of lateral researches and analysis of problems and prospects in such fields.