| 海藻糖-6-磷酸合成酶转基因烟草提高耐盐性的研究 |
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| 引用本文： | 郭蓓,,胡磊,何欣,陈雪梅,蒋湘宁. 海藻糖-6-磷酸合成酶转基因烟草提高耐盐性的研究[J]. 植物学报, 2008, 25(1): 41-49 |
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| 作者姓名： | 郭蓓 胡磊 何欣 陈雪梅 蒋湘宁 |
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| 作者单位： | 1北京林业大学生物科学与技术学院，北京 100083；2北京农学院生物技术系, 北京 102206 |
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| 基金项目： | 国家自然科学基金,北京市高校人才强教计划 |
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| 摘 要： |
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| 关 键 词： | 基因克隆与表达 定量PCR 胁迫耐受 转基因植物 TPS |
| 收稿时间： | 2007-06-01 |
| 修稿时间： | 2007-07-10 |
Trehalose-6-phosphate Synthase Transgenic Tobacco Enhanced Tolerance to Salt Stress |
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| Bei Guo,,Lei Hu,Xin He,Xuemei Chen,Xiangning Jiang. Trehalose-6-phosphate Synthase Transgenic Tobacco Enhanced Tolerance to Salt Stress[J]. Bulletin of Botany, 2008, 25(1): 41-49 |
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| Authors: | Bei Guo Lei Hu Xin He Xuemei Chen Xiangning Jiang |
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| Affiliation: | 1College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China; 2Department of Biotechnology, Beijing University of Agriculture, Beijing 102206, China |
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| Abstract: | Trehalose-6-phosphate synthase(TPS), which plays an important role in the synthesis of trehalose has received much attention in recent years. Research into TPS has mainly focused on bacteria and fungi. However, little research in plants has been reported. This paper describes the cloning and functional analysis of TPS from Arabidopsis. The Arabidopsis cDNA sequence of TPS in the pET30a(+) vector was heterogeneously overexpressed in BL21 of E. coli. Then, TPS was cloned into the plant expression vector pBI121 and transformed into tobacco. The expression of target gene in the transgenic tobacco plants was confirmed with real-time PCR. The transgenic tobacco line S5 showed higher trehalose level, which enhances the tolerance of transgenic plant to salt stress. |
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| Keywords: | ; |
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