Translation of a histone H3 tail as a model system for studying peptidyl-tRNA drop-off |
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Authors: | Kang Taek Jin Suga Hiroaki |
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Affiliation: | Department of Chemical and Biochemical Engineering, Dongguk University-Seoul, Seoul, South Korea. taekkang@dongguk.edu |
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Abstract: | We found that the synthesis of histone H3 N-terminal peptide (tail) in a reconstituted protein synthesis system yielded fragmented peptides along with the full-length product. With the combined use of MALDI-TOF analysis and peptidyl-tRNA hydrolase cleavage of the Flag tagged product species, we concluded that the fragments were generated by peptidyl-tRNA drop-off at specific sites and subsequent translation continuation. Using the histone H3 tail we also found that peptidyl-tRNA drop-off is strongly correlated with the amino acid context. We envision that the system described here would be useful as a model system for studying peptidyl-tRNA drop-off events. |
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