崇左金花茶花朵和叶片类黄酮UPLC-Q-TOF-MS分析

以崇左金花茶（Camellia chuangtsoensis）为材料，利用超高效液相色谱-四极杆-飞行时间质谱（UPLC-Q-TOF-MS）联用技术定性定量分析其花朵（花瓣、雄蕊）和叶片（老叶、新叶）中类黄酮成分与含量。结果表明，崇左金花茶中共检测到14种类黄酮成分，木犀草素、木犀草素-7-O-芸香糖苷、槲皮素-3，7-O-二葡萄糖苷、芸香柚皮苷、圣草素和染料木苷为山茶属金花茶组植物中首次发现，其中槲皮素-3，7-O-二葡萄糖苷、芸香柚皮苷、圣草素和染料木苷主要存在于花朵中，木犀草素和木犀草素-7-O-芸香糖苷在花朵中含量高于叶片，雄蕊中高于花瓣；槲皮素-3-O-葡萄糖苷、槲皮素-7-O-葡萄糖苷、槲皮素-3-O-芸香糖苷和山柰酚-3-O-葡萄糖苷为金花茶组植物叶片中首次发现，其叶片中含量远低于花朵，老叶中远低于新叶，雄蕊中远低于花瓣；儿茶素和表儿茶素在花朵中含量高于叶片，雄蕊中高于花瓣；槲皮素和山萘酚在花朵和叶片中含量均较低。崇左金花茶花瓣和雄蕊中含量较高的类黄酮为儿茶素类、木犀草素类和槲皮素类，主要是表儿茶素、木犀草素和槲皮素-3-O-葡萄糖苷；叶片中为儿茶素类和木犀草素类，主要是表儿茶素、木犀草素和木犀草素-7-O-芸香糖苷。崇左金花茶花瓣和雄蕊中儿茶素类、木犀草素类及类黄酮总量均高于叶片，且雄蕊高于花瓣；花瓣和雄蕊中槲皮素类远高于叶片，且花瓣中远高于雄蕊。

UPLC-Q-TOF-MS Analysis of Flavonoids in Flowers and Leaves of Camellia chuangtsoensis

The components and contents of flavonoids in flowers including petals and stamens and leaves including old leaves and new leaves of Camellia chuangtsoensis were qualitatively and quantitatively analyzed by ultra-performance liquid chromatography quadrupole-time-of-flight mass spectrometry. The results showed that fourteen flavonoids were detected in C.chuangtsoensis. The flavonoids in cluding luteolin, luteolin-7-O-rutinoside, quercetin-3, 7-O-diglucoside, narirutin, eriodictyol and genistin were identified in section Chrysantha Chang of Camellia for the first time, and quercetin-3, 7-O-diglucoside, narirutin, eriodictyol and genistinwere mainly found in the flowers. The contents of luteolin and luteolin-7-O-rutinosidein flowers were higher than thosein leaves, and the content of them in stamens were higher thanthose in petals. Quercetin-3-O-glucoside, quercetin-7-O-glucoside, quercetin-3-O-rutinoside and kaempferol-3-O-glucoside were identified in leaves of section Chrysantha of Camellia for the first time, and the contents of them in leaves were far lower than that in flowers. The contents of the four flavonoids in old leaves were far lower than that in new leaves, and the contents of them in stamens were far lower than that in petals. The contents of catechin and epicatechin in flowers were higher than those in leaves, and the content of them in stamens were higher than that in petals. The contents of quercetin and kaempferol in flowers and leaves were very low. The flavonoids with higher contents in petals and stamens of C.chuangtsoensis were catechins, luteolins and quercetins, which mainly included catechin, luteolin and quercetin-3-O-glucoside. The flavonoids with higher contents in leaves were catechins and luteolins, which mainly includedcatechin, luteolin and luteolin-7-O-rutinoside. The contents of catechins, luteolins and total flavonoids in petals and stamens were higher than that in leaves, and the content of them in stamens were higher than that in petals. The contents of quercetins in petals and stamens were far higher than that in leaves, and the contents of them in petals were higher than that in stamens.