小黑杨基因组的初步组装及SSR信息分析

小黑杨是人们以小叶杨和欧洲黑杨为亲本培育出的杂交种，兼具双亲生长速度快、抗性强的优点。本研究通过二代测序技术，对小黑杨进行全基因组测序，初步组装出小黑杨基因组，并以此为基础，识别分析其SSR序列，为小黑杨品种划分、表型性状关联等提供参考。结果表明，共组装得到了366 876条总计368.96 Mbp的contig序列；对其中不小于2 000 bp的21 788条的非冗余contig进行SSR分析，共识别出18 111条SSR序列，其中一、二、三核苷酸重复基序较多。对得到的SSR序列设计引物，共得到12 838对引物，供今后实验使用。

Sequencing and Assembly of Populus simonii×P.nigra Genome and SSR Analysis

Populus simonii×P.nigra, which is the hybrid crossed by P.simonii and P.nigra, inherits the advantages of fast growth and strong resistance from their parents. In this study, the genome sequence of Populus simonii×P.nigra was preliminarily sequenced and assembled by the next generation sequencing(NGS) technology. The SSR sequences were identified and analyzed. We hope this study can provide a reference for classification and phenotypic correlation of Populus simonii×P.nigra. The results show that we total assembled 368.96 Mbp genome sequence, which contain 366 876 contigs. SSR analysis was performed on 21 788 non-redundant contigs which were not less than 2 000 bp. In total, 18 111 SSR sequences were identified, and most of them are one, two or three nucleosides acid repeat motif. The primers were designed for the obtained SSR sequences, and a total of 12 838 primers were obtained for future experiments.