Basement membrane heparan sulfate proteoglycan from the L2 rat yolk sac carcinoma |
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Authors: | M Fenger U Wewer R Albrechtsen |
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Institution: | 1. Laboratoire de Physiologie Cellulaire Végétale, CNRS ERA 488, Université Scientifique et Médicale de Grenoble, BP 68, F-38402 Saint Martin d''Héres Cédex, France;2. Laboratoire de Biochimie, Département de Biologie Moléculaire, Institut Pasteur, 25 rue du Dr Roux, F- 75724 Paris Cédex 15, France |
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Abstract: | Heparan sulfate proteoglycan from the L2 rat yolk sac carcinoma has been purified and partially characterized. The proteoglycan has an apparent Mr of 750 000, 35% of which represents the core protein. The core protein seems to be homogeneous, whereas the heparan sulfate chains are heterogeneous with an Mr of about 50 000-70 000, with 30% of the glucosamine being N-sulfated. Antibodies raised against the core protein of the heparan sulfate proteoglycan reacted with basement membranes of various rat and human tissue. |
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Keywords: | Histone-like protein Chloroplast Phylogeny Higher plant Escherichia coli Synechocystis |
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