Effect of redox mediators on the flash-induced membrane potential generation in Mn-depleted photosystem II core particles |
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Authors: | Oksana A Gopta Anna A Tyunyatkina Vasiliy N Kurashov Alexey Yu Semenov Mahir D Mamedov |
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Institution: | (1) A.N. Belozersky Institute of Physical-Chemical Biology, Moscow State University, Vorob’evy gory, GSP-2, 119991 Moscow, Russia |
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Abstract: | An electrometrical technique was used to investigate flash-induced electron transfer reactions between Mn-depleted spinach
photosystem II core particles incorporated into liposomes and redox mediators. Besides the fast increase in the transmembrane
electric potential difference associated with electron transfer between the redox active tyrosine (YZ) and the primary quinone acceptor QA, an additional electrogenic phase was observed in the presence of N,N,N′N′-tetramethyl-p-phenylenediamine and 2,6-dichlorophenol-indophenol. The latter phase is attributed to vectorial electron transfer from the
redox dye(s) to the protein-embedded YZ. The data obtained suggest an electrically isolated location of the YZ from the external water phase. |
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Keywords: | Mn-depleted photosystem II Proteoliposomes Redox mediators Photoelectric response Electrometrical technique |
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