In vivo haploid induction in maize |
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| Authors: | J Eder S Chalyk |
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| Institution: | 1.Bavarian State Institute for Agronomy, V?ttinger Strasse 38, 85316 Freising, Germany e-mail: joachim.eder@lbp.bayern.de Tel.: +49 8-161 7-13633, Fax: +49 8-161 7-14305,DE;2.Institute of Genetics, Padurii 20, Chisinau 2002, Moldova,MD |
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| Abstract: | Two haploid-inducing lines, MHI and M741H, were used for the production of maternal haploids. Haploids were obtained from
all maternal genotypes involved in the experiment, including dent, flint and flint×dent maize. The maternal genotype had a
significant influence on the frequency of haploids obtained. The frequency ranged from 2.7% to 8.0%. For chromosome-doubling
seedlings were treated with colchicine solution, and 49.4% of the haploid plants produced fertile pollen, 39.0% could be selfed
and 27.3% produced seeds after selfing. Synthetic populations, improved by haploid sib recurrent selection, were tested in
a field trial. The results show that the utilization of maternal haploid plants has great potential for maize breeding and
maize genetics.
Received: 14 May 2001 / Accepted: 3 August 2001 |
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| Keywords: | In vivo haploids Haploid-inducing lines Chromosome doubling Haploid sib recurrent selection |
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