小麦遗传型与生理型雄性不育花药蛋白质双向电泳分析

为了研究小麦雄性不育蛋白表达机制, 以具有异质同核的3个亲本: 即遗传型不育系ms(S)-西农1376、对应的保持系(A)-西农1376和生理型(化学杀雄剂SQ-1诱导)雄性不育系ms(A)-西农1376为材料, 利用IEF/SDS-PAGE凝胶电泳技术, 对发育到单核后期的花药全蛋白特异性进行了比较分析, 得到320~350个清晰的蛋白点。结果表明: 遗传型和SQ-1诱导的生理型不育系蛋白质图谱与正常保持系在蛋白质(多肽)表达量上存在一定的差异, 同时发现有几种蛋白质的表达有明显的特异性, 两个不育材料2D胶上共有几个明显的特异蛋白点, 而在保持系中未发现; 两个不育材料又分别具有自己的特异蛋白表达, 不育机理不同; 比较分析可知, 不育系中某些蛋白的表达受到了抑制, 并开启了与花药败育有关的特定蛋白的表达, 可能使物质能量代谢受阻,导致雄性不育的发生。

Two-dimensional electrophoresis on anther total proteins of male sterility induced by chemical hybridizing agent and heredity type in wheat

The isonucleus-allocytoplasmic materials ms(S)-Xinong 1376, (A)-Xinong 1376, and ms(A)-Xinong 1376 were used to investigate the protein expression profile in mononucleated anaphase anthers of wheat (Triticum aestivum L.) after silver staining and analysis by PDQuest software. About 320 to 350 distinct protein spots were detected reproducibly. The results indicated that there were some difference in the protein quantity between the two sterile lines and the maintainer line. Sev-eral specific spots were present in the maps of two sterile lines, but were absent in the maintainer line. The two sterile lines had their own specific expression protein spots with different sterile mechanism. We compared the three maps and con-cluded that the sterile lines may inhibit related protein expression, and turned on some proteins related to male sterility, which disturbs the normal metabolism of substances and energy in anthers and leads to the occurrence of male-sterility.