Construction of an arabidopsis BAC library and isolation of clones hybridizing with disease-resistance,gene-like sequences |
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| Authors: | Guo-Liang Wang Randy Warren Goger Innes Brian Osborne Barbara Baker Pamela C. Ronald |
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| Affiliation: | (1) Department of Plant Pathology, University of California at Davis, 95616 Davis, CA, USA;(2) Department of Biology, Indiana University, 47405 Bloomington, IN, USA;(3) Plant Gene Expression Center, USDA, 94710 Albany, CA, USA |
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| Abstract: | A bacterial artificial chromosome (BAC) library consisting of 9,000 clones (representing a 4.5 genome equivalents) with an average DNA insert size of 60 kb was constructed from arabidopsis nuclear DNA. We have demonstrated the usefulness of this library by identifying one BAC clone that hybridizes with the arabidopsisPHYB gene and seven clones, representing four distinct classes, that hybridize to a putative disease-resistance gene. This library represents an additional resource for map-based cloning and physical mapping in arabidopsis. |
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| Keywords: | BAC map-based cloning chromosome walking disease-resistance genes |
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