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| 双价抗病基因导入籽瓜的遗传转化研究 | |
| 引用本文： | 辛莉,施江,乐锦华,郑学勤,陆璐,王登伟. 双价抗病基因导入籽瓜的遗传转化研究[J]. 生物技术, 2005, 15(2): 16-19 |
| 作者姓名： | 辛莉 施江 乐锦华 郑学勤 陆璐 王登伟 |
| 作者单位： | 1. 河南科技大学,河南,洛阳,471003 2. 石河子大学生物工程学院,新疆,石河子,832003 3. 中国热带农业科学院热带生物技术国家重点实验室,海南,海口,571101 |
| 摘 要： | 以籽瓜主栽品种新籽瓜2号、五原籽瓜等为研究材料，用苗龄1-3d的子叶切块作外植体，不定芽诱导培养基为MS 6-BA2mg/L IBA 0.1mg/L，芽伸长培养基为MS KT0．2mg／L，诱导生根的培养基是MS IAA0．5mg／L。籽瓜遗传转化研究结果表明农杆菌感染时间以10min，共培养时间以2d为合适。共获得抗卡那霉素100mg／L的试管苗41个，PCR检测呈阳性的有15株，转化率为34．9％。该研究初步建立了籽瓜的遗传转化体系和转化体的检测体系。 |
| 关 键 词： | 籽瓜 植株再生 遗传转化 PCR |
| 文章编号： | 1004-311X(2005)02-0016-04 |
| 修稿时间： | 2004-11-25 |
Research of Genetic Transformation in Seed Watermelon |
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| XIN Li,SHI Jiang,LE Jin-hua,ZHENG Xue-qin,LU Lu,WANG Deng-wei. Research of Genetic Transformation in Seed Watermelon[J]. Biotechnology, 2005, 15(2): 16-19 | |
| Authors: | XIN Li SHI Jiang LE Jin-hua ZHENG Xue-qin LU Lu WANG Deng-wei |
| Affiliation: | XIN Li~1,SHI Jiang~1,LE Jin-hua~2,ZHENG Xue-qin~3,LU Lu~3,WANG Deng-wei~3 |
| Abstract: | The research of genetic transformation in seed watermelon has not been reported.The research materials were Xinzigua No.2,Wuyuanzigua etc.,by using the basal and middle segments of cotyledon for young plants of 1 to 3 days age as the explant.The medium for callus and adventitious shoot induction was MS 6-BA 2mg/L IBA 0.1mg/L.The medium for shoot elongation was MS KT0.2mg/L.The medium for root induction was MS IAA 0.5mg/L.The transformation experiment suggested that the best treatment of inoculation by soaking for 10 min,and cocultivation in darkness for 2 days.41 plantlets were obtained after selection on 100 mg/L kanamysin.The kanamysin-resistant plantlets were verified by PCR analysis,fifteen of them was proved to be of transgenic plants. |
| Keywords: | Seed watermelon plant regeneration genetic tranformation polymerase chain reaction |
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