Regional appearance of atrial natriuretic peptide in the ventricles of infarcted rat hearts |
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Authors: | Terje H Larsen Thorvald Sætersdal |
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Institution: | 1. Cellular Cardiology Research Group, Department of Anatomy and Cell Biology, University of Bergen, ?rstadveien 19, N-5009, Bergen, Norway
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Abstract: | The appearance of atrial natriuretic peptide (ANP) in the ventricular myocardium was investigated in rat hearts subjected
to severe left ventricular infarction. The left coronary artery was ligated for 1, 2, 3, 4 and 6 days and for 3 weeks, and
the tissue was prepared for microscopic examination of immunoreactive ANP and for electron microscopy. In the normal and sham-operated
hearts, and in hearts subjected to 1 day of coronary ligation, ANP immunoreactivity was restricted to a few ventricular myocytes
of the conduction system. Following 2–3 days of coronary ligation, ANP immunoreactivity was detected in the viable myocardium
of the lateral border of the infarct and in a few layers of viable cardiac myocytes located in the subendocardial areas of
the ischemic left free ventricular wall. Further, during the following days and after 3 weeks of coronary ligation, a gradient
of specific labeling was commonly seen across the lateral border area of the infarct. Thus, the strongest immunoreactivities
were present in the cardiac myocytes located adjacent to the non-contracting myocardium. Electron microscopic examination
of the immunoreactive cardiac myocytes confirmed the presence of electron-dense specific granules within these cells. The
present findings suggest that the increased regional production of ANP within the ventricular myocardium is induced by increased
mechanical stretch of the cardiac myocytes, and that this might contribute to the increased release of ANP in myocardial infarction. |
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Keywords: | Atrial natriuretic peptide Specific granules Cardiac myocytes Myocardial infarction Immunocytochemistry |
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