| Abstract: | Serum-derived albumin has for a long time been used in cell culture media, but the exact role of albumin and/or impurities
bound to albumin has not been precisely defined. In this study, recombinant human albumin was evaluated for its growth-promoting
activity on two cell lines, NRK and SCC-9. For NRK cells, the recombinant human albumin was found to exert an inhibitory effect.
The fact that fatty acid free HSA was also inhibitory while HSA fraction V was stimulatory suggested a role for fatty acids
or some other bound moieties in growth stimulation by HSA fraction V. Addition of oleic acid, cholesterol, phosphatidylcholine,
phosphatidylserine or a combination of these lipids, however, did not significantly improve the growth stimulating activity
of either fatty acid free HSA or the recombinant human albumin. For SCC-9 cells, both recombinant human albumin and fatty
acid free HSA showed slight stimulation (although they were not as active as HSA fraction V), suggesting that in some cell
systems, the albumin molecule per se may promote cell growth and survival.
This revised version was published online in July 2006 with corrections to the Cover Date. |
