Oxysterols induce apoptosis and accumulation of cell cycle at G(2)/M phase in the human monocytic THP-1 cell line |
| |
Authors: | Lim Hee-Kyoung Kang Hee-Kyoung Yoo Eun-Sook Kim Bum-Joon Kim Youn-Woo Cho Moonjae Lee Jae-Hwa Lee Yun-Sil Chung Myung-Hee Hyun Jin-Won |
| |
Affiliation: | Department of Biochemistry, College of Medicine Cheju National University, 1 Ara 1-dong, Jeju, Jeju-do, 690-756, South Korea. |
| |
Abstract: | The cytotoxic activity of oxysterols, 7 beta-hydroxycholesterol (7 beta-OHC) and 25-hydroxycholesterol (25-OHC), has been evaluated using various leukemia cell lines. Among the tested cell lines, both oxysterols showed the highest cytotoxicity to THP-1, human monocytic leukemia cell line. These oxysterols induced apoptosis through down-regulation of Bcl-2 expression and activation of caspases. Also, the oxysterols showed the accumulation at G(2)/M phase of cell cycle through down-regulation of cyclin B1 expression. Taken together, these results indicated that both 7 beta-OHC and 25-OHC inhibited the proliferation of THP-1 cells through apoptosis and cell cycle accumulation at G(2)/M phase. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|