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拟南芥AtcpSecA基因表达的特异性
引用本文:李金萍,李鹏丽,于蕴卿,王宁宁.拟南芥AtcpSecA基因表达的特异性[J].植物生理学通讯,2009(3):244-248.
作者姓名:李金萍  李鹏丽  于蕴卿  王宁宁
作者单位:南开大学生命科学学院,天津300071
基金项目:国家“863”项目(2007AA102105)、国家自然科学基金项目(30670193)和教育部“新世纪优秀人才支持计划”项目(NCET-05-0224).
摘    要:前期研究表明AtcpSecA基因的突变使叶绿体发育缺陷,内部缺少正常类囊体片层结构,叶片呈黄白色。在此基础上我们进一步研究AtcpSecA基因的表达特异性,并构建了AtcpSecA基因启动子与报告基因GUS的融合基因AtcpSecA::GUS,以农杆菌介导方法转化获得转基因拟南芥。GUS组织化学染色结果表明,在AtcpSecA::GUS转基因拟南芥的下胚轴、子叶、叶片、果柄等绿色组织中有很强的GUS活性,而在根、花序和种荚等非绿色组织中几乎没有GUS活性。降低培养基中琼脂浓度转基因拟南芥中AtcpSecA::GUS基因的表达明显受抑制,暗中则显著受到促进。

关 键 词:拟南芥  AtcpSecA基因  启动子  表达特异性

Expressional Characterization of Arabidopsis AtcpSecA Gene
LI Jin-Ping,LI Peng-Li,YU Yun-Qing,WANG Ning-Ning.Expressional Characterization of Arabidopsis AtcpSecA Gene[J].Plant Physiology Communications,2009(3):244-248.
Authors:LI Jin-Ping  LI Peng-Li  YU Yun-Qing  WANG Ning-Ning
Institution:( College of Life Sciences, Nankai University, Tianjin 300071, China)
Abstract:We identified an Arabidopsis mutant with a T-DNA insertion in AtcpSecA gene. The mutant of AtcpSecA gene resulted in chloroplast development defects. The homozygous mutant lacked thylakoid lamella and showed albino phenotype. In order to study AtcpSecA gene expression pattern, we cloned the promoter of AtcpSecA gene and an expression vector containing the AtcpSecA::GUS fusion gene was constructed and introduced into Arabidopsis by Agrobacterium-mediated floral dip method. The strong GUS expression were observed in hypocotyls, cotyledon, leaves and pedicels, while weak GUS expression were observed in roots, inflorescence and siliques. The expression of AtcpSecA::GUS was suppressed when agar concentration was decreased, and dark treatment could increase the expression of AtcpSecA::GUS.
Keywords:Arabidopsis  AtcpSecA gene  promoter  expression specificity
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