麻疯树中ADP核糖基化因子基因的克隆和表达

从麻疯树cDNA文库中筛选到包含完整编码序列的ADP核糖基化因子的cDNA序列，命名为Jc-arf．其长度为887bp，开放阅读框546bp，推测的编码蛋白含181个氨基酸残基，具有典型的GTP结合蛋白家族的特点：P框（GLDAAGKT）、G’框（DVGGQ）和G框（NKQDL）。序列分析显示，Jc-arf矿接近于人类ClassI型arf基因，其蛋白序列与多种植物ARF有很高的同源性。半定量RT-PCR结果显示，Jc-arf的表达具有一定的组织特异性，在花中最丰富。PEG、低温、NaCl胁迫下，Jc-arf的表达受PEG和低温的诱导，而对NaCl胁迫不敏感。

Cloning and Expression of an ADP-ribosylation Factor Gene in Jatropha curcas L.

An ADP-ribosylation factor gene, named Jc-arf, was cloned from Jatropha curcas. The cloned eDNA included a 546 bp complete open reading frame, which encoded a 181 amino acids peptid. This putative peptid contained three motifs： GLDAAGKT, DVGGQ and NKQDL, which involved in the feature of GTP-binding proteins. The alignment results showed Jc-ARF was high identity with many ARFs in other plants, and it was similar to ClassI of human ARFs. The semi-quantitative PCR results showed that Jc-arfexpression was the highest in flowers. Further, the expression of Jc-arf could be induced in roots, stems and leaves by PEG, in roots under cool stress. However, it seemed insensitive to the NaCl stress.