| 小麦成熟胚脱分化过程CDPKs基因的差异表达分析 |
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| 引用本文： | 雷志华,;崔琰,;赵一丹,;陈军营,;陈新建.小麦成熟胚脱分化过程CDPKs基因的差异表达分析[J].植物生理学通讯,2009(2):125-128. |
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| 作者姓名： | 雷志华 ;崔琰 ;赵一丹 ;陈军营 ;陈新建 |
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| 作者单位： | [1]河南农业大学农学院,郑州450002; [2]郑州师范高等专科学校生命科学系,郑州450044 |
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| 基金项目： | 国家转基因植物研究与产业化专项基金（JY03-B-19-2）和河南省杰出人才创新基金（022100090）. |
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| 摘 要： | 用Affymetrix小麦基因芯片检测小麦成熟胚脱分化过程中CDPKs基因的表达变化的结果表明，在检测到的16个CDPKs基因中，有15个基因在小麦成熟胚脱分化过程中发生了有意义变化，其中上调表达基因有12个，上调2-7．5倍，下调表达基因有2个，下调3娟．1倍，在不同时点既有上调又有下调的1个。根据已知CDPKs生物功能扣它们表达变化趋势以及这些变化趋势与脱分化过程中的吻合程度分析结果，推测CPK2A、CPK2B．CPK6和Os12g0169800基因参与了小麦成熟胚脱分化过程的启动和愈伤组织的形成。
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| 关 键 词： | 蛋白激酶 CDPKs 基因芯片 脱分化 |
Analysis of Different Expression of CDPKs Genes in the Dedifferentiation of Mature Wheat Embryo |
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| Institution: | LEI Zhi-Hua, CUI Yan, ZHAO Yi-Dan, CHEN Jun-Ying, CHEN Xin-Jian (1. College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China; 2.Life Sciences Department, Zhengzhou Teachers College, Zhengzhou 450044, China) |
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| Abstract: | Expression profiles of CDPKs genes were analyzed by Affymetrix Microarray technique during wheat mature embryo dedifferentiation. Out of 16 CDPKs genes detected by microarray, 15 genes were significantly changed in expression pattern during dedifferentiation of mature wheat embryo. Furthermore, 12 genes were up-regulated by 2-7.5 times, 2 genes were down-regulated by 3-6.1 times, and 1 gene was up- or downregulated at the different stages of dedifferentiation. According to the known functions of CDPKs, their changed trend and the coincidence of the trend and the dedifferentiation degree, it was predicted that CPK2A, CPK2B, CPK6 and Os12gO169800 might play roles in the initiation of dedifferentiation of mature wheat embryos and the formation of calli. |
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| Keywords: | protein kinases CDPKs gene microarray dedifferentiation |
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