柠条锦鸡儿肌动蛋白基因的克隆和表达稳定性分析

以几种豆科植物中肌动蛋白的氨基酸保守序列设计简并引物，采用RT—PCR结合RACE扩增技术，从柠条锦鸡儿叶片中克隆到一个编码肌动蛋白的基因，命名为CkACT。该基因cDNA全长为1655bp，开放阅读框1134bp，编码377个氨基酸。氨基酸比对分析表明，该基因编码的氨基酸与其他植物肌动蛋白基因具有较高的同源性。不同组织中的表达基本上一致，不同发育时期的基因表达相似，低温、NaCl、干旱和ABA处理后的表达强度没有明显差异，说明CkACT基因表达稳定。

Clone and Expression Stability Analysis of Actin Gene in Caragana korshinskii

An actin gene, CkACT, was cloned from the leaves of Caragana korshinskii by using RT-PCR and RACE with degenerate primers which were synthesized based on the high homologous region among the sequences of several leguminous ACT genes. The full-length cDNA of CkACT was 1 655 bp and contained a 1 134-bp open reading frame encoding a 377-amino acid protein. The deduced amino acid sequence of CkACT protein shared high identity with other ACTINs via multiple alignment. CkACT expressed constitutively in all tested organs, including root, shoot and leaf. There was no significant difference in mRNA expression in different development stage or under cold, NaCl, drought and ABA treatment. These results indicated that the expression of CkACT is stable.