Generation and Imaging of Transgenic Mice that Express G-CaMP7 under a Tetracycline Response Element |
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| Authors: | Masaaki Sato Masako Kawano Masamichi Ohkura Keiko Gengyo-Ando Junichi Nakai Yasunori Hayashi |
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| Affiliation: | 1RIKEN Brain Science Institute, Wako, Saitama, Japan;2PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama, Japan;3Brain Science Institute, Saitama University, Saitama, Japan;Tokyo University of Agriculture, JAPAN |
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| Abstract: | The spatiotemporally controlled expression of G-CaMP fluorescent calcium indicator proteins can facilitate reliable imaging of brain circuit activity. Here, we generated a transgenic mouse line that expresses G-CaMP7 under a tetracycline response element. When crossed with a forebrain-specific tetracycline-controlled transactivator driver line, the mice expressed G-CaMP7 in defined cell populations in a tetracycline-controlled manner, notably in pyramidal neurons in layer 2/3 of the cortex and in the CA1 area of the hippocampus; this expression allowed for imaging of the in vivo activity of these circuits. This mouse line thus provides a useful genetic tool for controlled G-CaMP expression in vivo. |
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