马桑内酯对粘虫幼虫体内3种酶活性的影响

采用3种马桑内酯——羟基马桑毒素、马桑亭和马桑宁饲喂和注射粘虫幼虫,测定和分析了虫体组织中乙酰胆碱酯酶(AChE)、谷胱甘肽S-转移酶(GST)和多酚氧化酶(PPO)活性变化.结果表明,3种马桑内酯对试虫体内乙酰胆碱酯酶活性的影响存在差异,在4~48 h大多时间段具有激活特性;而谷胱甘肽S-转移酶的活性或被激活或被抑制,饲喂用药后4~8 h中肠组织中酶被激活,12 h酶活性被抑制,整个过程呈现S型;多酚氧化酶活性受3种毒素的影响不明显.

Effect of Coriaria Lactone on the Activities of Three Enzymes in Mythimna separata

The effect of Tutin,Coiriatin and Corianin on acetylcholinesterase(AChE),glutathione S-transferase(GST) and Polyphenol Oxidase(PPO) of Mythimna separata larvae were studied.The results indicated the changes of AChE activities were different among the three toxins.The AChE was activated in most cases during 4~48 h.The activities of GST were enhanced or inhibited in different tissues and different times.The enzyme was activated in midgut with feeding treatment after 4~8 h and was inhibited after 12 h.The changes of activity showed S curve.The activities of PPO was not significantly different after the three toxins were used.